Introduction to enzyme kinetics. Explore enzyme kinetics and how enzymes speed up reactions by reducing activation energy. Learn about the two-step process of the enzyme. The rate of a reaction controlled by an enzyme is mainly influenced by: temperature, substrate concentration, enzyme concentration and pH. By increasing the substrate concentration, the chance of a substrate colliding with an enzyme and subsequently reacting increases because there is simply more substrate moving around in the solution. Materials: 3 hydrogen peroxide Distilled water filtered water if done at home Liquid dish soap Test tubes Potatoes or liver source of catalase Water bath ml amp, syringe Procedure: Prepare a different concentration of the substrate Hydrogen peroxide. diluting hydrogen peroxide results in different. At low product concentrations, such as \\widetilde P 0.1\, the lower substrate concentration defines the preference for the binder Fig. 3b. Likewise, at high product concentrations. A group of students monitored the substrate concentration during an enzyme-controlled reaction. Select the graph that correctly shows how the substrate concentration changes during the course of the reaction. The concentration of the substrate will decrease over time as it is converted into productCooperativity. Explore the intriguing world of enzyme kinetics and cooperative binding. Learn more about the two-step process of enzyme catalysis, the Michaelis Menten equation, and the effects. The equation describes the dependence of enzyme-catalyzed reaction rates on the substrate concentration. W. amp Schnell, S. On the estimation errors of km ev from the time course.